抗性选育协同疫苗免疫对鸡羽囊马立克病毒载量的影响

doi:10.11843/j.issn.0366-6964.2015.05.021

Abstract

Abstract:

The aim of the present study was to study the effects of HVT-vaccinated and MD resistant selection on virus loads in feather follicle of chickens after the challenge with MDV-1.Real-time FQ-PCR was used for absolute quantification of the DNA levels of meq of feather follicle sampled in 5 different times（7，14，21，28，35 DPI） from all chickens after the challenge for quantification of MDV-1.The load of MDV-1 was detected in feather follicle to analyzed and compared proliferation of MDV-1 in resistant/common or/and HVT-vaccinated/HVT-unvaccinated chickens.The results showed that 1） from the impact of MD-resistant on the MDV-1 loads，the load of MDV-1 were significantly higher in chickens of F group（common line，HVT-unvaccinated and MDV-challenged） whereas significantly lower（P<0.05） in chickens of T group（MD-resistant line，HVT-unvaccinated and MDV-challenged） in 14，21 DPI；On the one hand，R group（MD-resistant line，HVT-vaccinated and MDV-challenged） and G group（common line，HVT-vaccinated and MDV-challenged） give about the same load of MDV-1；2） From the impact of HVT-vaccinated on the MDV-1 loads，the load of MDV-1 were significantly higher in chickens of T group（MD-resistant line，HVT-unvaccinated and MDV-challenged） whereas significantly lower（P<0.05） in chickens of R group（MD-resistant line，HVT-vaccinated and MDV-challenged） in 28 DPI；On the one hand，the load of MDV-1 were significantly higher in chickens of F group（common line，HVT-unvaccinated and MDV-challenged） whereas significantly lower（P<0.05）in chickens of G group（common line，HVT-vaccinated and MDV-challenged） in 14，21 DPI：3） From the impact of MD-resistant and HVT-vaccinated on the MDV-1 loads，the load of MDV-1 were significantly higher in chickens of F group（common line，HVT-unvaccinated and MDV-challenged） whereas significantly lower in chickens of R group（MD-resistant line，HVT-vaccinated and MDV-challenged） in 14（P<0.05），28（P<0.01） and 35（P<0.05）DPI.These results indicated that the virus loads in feather follicle of chickens can be reduced significantly by resistance selection in coordination with vaccination，which will reduce the risk of MDV-1 transmission and improve the chicken’s chance of survival.

CLC Number:

S852.659.1

JIN Yuan-chang，CHEN Zhuang，LI Yu-feng，YUAN Zhi-dong . Effects of Resistance Selection in Coordination with Vaccination on MDV-1 Loads in Feather Follicle of Chickens[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(5): 836-840.

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Effects of Resistance Selection in Coordination with Vaccination on MDV-1 Loads in Feather Follicle of Chickens

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